Effect of Culture Conditions on L-Dopa Accumulation in Callus Culture Of Mucuna pruriens
ABSTRACT Effect of various carbon and nitrogen sources, PGRs and their precursors, on the callus growth and L-dopa production by the stem and leaf derived callus of Mucuna pruriens were studied. Five different carbon sources were glucose, fructose, sucrose, mannose and raffinose. Glucose and sucrose were almost equally effective in both promoting the growth as well as L-dopa content. The four nitrogen sources were phenylalanine, tyrosine, glutathione and casein hydrolysate.