Validation of Spectrophotometric Methods for Quanti tative Determination of 7-ADCA in Pharmaceutical Formulations
ABSTRACT Two simple, sensitive and accurate methods are described for the determination of 7-Amino deacetoxy cephalosporanic acid (7-ADCA) in bulk drug and in formulations. Method M 1 &M 2 are based on the oxidizing reaction between 7-ADCA and Ninhydrin-Ascorbic acid (NIN-AA) (M 1 ); β -Napthol-H 2 O 2 -Cupric nitrate-2, 4-DNP-Ammonium hydroxide ( β -NPT,H 2 O 2 -DNP)(M 2 ) solutions. The chromogen being extractable with chloroform could be measured quantitatively at 400 nm (M 1 ) and 420 nm (M 2 ).