Development, validation and application of UV spectrophotometric method for the determination of roxithromycin in bulk and pharmaceutical dosage form


ABSTRACT:

Roxithromycin is a semi-synthetic macrolide antibiotic. It is used to treat respiratory tract, urinary and soft tissue infections. The present research work discussed the development of a simple, sensitive, rapid, accurate, precise and economical UV Spectrophotometric method for the evaluation of Roxithromycin in bulk and pharmaceutical dosage form which is based on the measurement of absorption maxima at 420 nm. A Shimadzu 1800 U.V visible spectrophotometer with 1cm matched quartz cells, and de-ionized water as solvent were used. Developed methods obeyed the Beer’s law in the concentration range of 20-70μg/ml having line equation y = 0.020x + 0.168 with correlation coefficient of 0.999.Method was validated statistically. Percentage recovery of the drug for the proposed method ranged from (99.2280-99.5320 ±0.1670) indicating no interference of the capsule excipients. The developed method was validated with respect to precision, accuracy (recovery), linearity, limit of detection and limit of quantitation.

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For more details PDF Link: http://www.jocpr.com/articles/development-validation-and-application-of-uv-spectrophotometric-method-for-the-determination-of-roxithromycin-in-bulk-an.pdf


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