Isolation and screening of chitinase producing Serratia marcescens from soil
ABSTRACT:
In the present study an attempt was made to
isolate organisms that would synthesize chitinase which can be used for the
effective biodegradation of a vast majority of crustacean waste. Out of the
four isolates screened, the isolate I-3 showed maximum chitinase activity and
was selected for further research. The isolate I-3 was identified as Serratia
marcescens using the standard identification parameters like gram staining
followed by the biochemical assays. Further characterization and purification
of chitinase needs to be carried out.
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Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. chitinase
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