Selective and validated spectrophotometric methods for the determination of midazolam using N-Bromosuccinimide


ABSTRACT:

Simple and sensitive spectrophotometric methods for the determination of midazolam (MID) are proposed. The methods are based on the bromination of the drug using N-bromosuccinimide (NBS) and the excess oxidant is determined by either malachite green (MG) or safranin O (SO), the absorbances of which are measured at 617 or 522 nm for MID-MG and MID-SO, respectively. Under the optimized experimental conditions, Beer’s law is obeyed in the concentration ranges of 15.00-30.00 and 10.00-50.00 µgmL-1 with molar absorptivity of 8.88 × 103 and 1.18 × 104 L mol-1 cm –1 for methods A and B, respectively. The limits of detection for methods A and B are 1.7368 and 0.5823 g mL-1, respectively. No interference was observed from excipients commonly present in formulations. The proposed methods are applied successfully for the pharmaceutical formulations.

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