Evaluation of wound healing and chemotactic activities of Embelin and Vilangin using human dermal fibroblast (in vitro) model


ABSTRACT:

Plants or plant based products have been used in the traditional healthcare system from time immemorial for treatment of various diseases; wound healing is one among them. The aim of present study is to understand the 2, 5- dihydroxy-3-undecyl-1, 4-benzoquinone (Embelin) and dimeric form of embelin (Vilangin) mediated attenuation of wound healing and cell migration at the cellular level. We used fibroblast cell based model for studying cytotoxicity, wound healing and Boyden’s chamber migration assay. In addition to this, effect of cell migration of both embelin and vilangin was studied at the single cell level using live cell imaging technique. Results, shown that the concentration required for 50% cyotoxicity (IC50) towards human dermal fibroblast cells was determined as 32 ± 1 µg/ml & 15 ± 2 µg/ml for embelin and vilangin respectively. Furthermore 0.1 and 1.0 µg/ml concentration were chosen for further wound healing and migration studies. Results demonstrate that both embelin and vilangin arrests wound healing (at 0.1, 1.0 µg/ml concentration level individually) and moreover human dermal fibroblast (HDF) migration was significantly reduced after treatment with both embelin and vilangin respectively. Our present findings demonstrated both embelin and dimeric form of embelin (vilangin) inhibits wound healing and cell migration. Therefore both could be used as potential drug candidates for anti-angiogenesis therapy.

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For more details visit: http://www.jocpr.com/articles/evaluation-of-wound-healing-and-chemotactic-activities-of-embelin-and-vilangin-using-human-dermal-fibroblast-in-vitro-mo.pdf


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