Preparation and toxicity of protein-calcium complex with phosphorylated human-like collagen


ABSTRACT:

In order to bind more amount of calcium, human-like collagen (HLC) was first phosphorylated by reacting with D-Glucose 6-phosphate disodium salt hydrate, and then the phosphorylated HLC (PHLC) was chelated with CaCl2 to prepare the PHLC-Ca composite. The Optimum reaction condition for the PHLC-Ca was: pH 7.5, nCaCl2 : nHLC = 600, stirring for 1 h. The Cell Counting Kit-8 (CCK-8) assay indicates that HLC, PHLC and PHLC-Ca were safe to BHK21 and could stimulate the normal growth of BHK21.


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For more details to visit Article Link: http://www.jocpr.com/articles/preparation-and-toxicity-of-proteincalcium-complex-with-phosphorylated-humanlike-collagen.pdf


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