Screening and identification of a novel lipase producing Aspergillus niger Yz15 and optimization for lipase production


ABSTRACT:

A lipolytic fungus named Yz15 which produced extracellular lipase was isolated from the water and soil samples contaminated with oil. The 18S rRNA sequencing revealed it as a novel strain of Aspergillus niger. Lipases was particularly important due to the fact that they were used for different industrial processing. So in this study we carried out optimization of lipase production from Aspergillus niger Yz15 using statistical designs. The important factors influencing the lipase activity were identified by initial screening method of Plackett-Burman experimental design, which showed that olive oil, magnesium sulfate and initial pH were the significant variables (P<0.05). Box-Behnken design and response surface analysis were adopted to the further optimization. A maximum lipase activity of 4.55U/mL was obtained at 1.54% olive oil, 0.03% magnesium sulfate and initial pH 6.17. The the second order quadratic model for lipase production was capable of predicting the pratical fermentation with high determination coefficient (R2 ) values of 0.9842. The average lipase production was observed to increase by about 67% using these statistical techniques of optimization of media and culture conditions. ><0.05) Box-Behnken design and response surface analysis were adopted to the further optimization. A maximum lipase activity of 4.55U/mL was obtained at 1.54% olive oil, 0.03% magnesium sulfate and initial pH 6.17. The the second order quadratic model for lipase production was capable of predicting the pratical fermentation with high determination coefficient (R2 ) values of 0.9842. The average lipase production was observed to increase by about 67% using these statistical techniques of optimization of media and culture conditions.

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