Quantification of bacterial biomass by DNA analysis

ABSTRACT:

Quantification of microbial biomass is very important for proper understanding of the microbial process in environment. There are many different methods available to estimate the bacterial biomass but they all suffer from some limitations. Due to the recent development in molecular methods, it is possible to quantify the bacterial biomass by analyzing the genomic DNA. The aim of this research work was to estimate the bacterial biomass by quantification of DNA extracted from halophilic bacterial isolates during the growth. For this purpose, two bacterial strains JSO1 and JSO2 isolated from halophylic environment were used. The strains isolated were sequenced and identified as Micrococcus lylae (JSO1) and Micrococcus luteus (JSO2). After monitoring the growth, the cultured samples were used for extraction of DNA and were analyzed by UV spectrophotometer. JSO1 showed 13µg/ml growth compared to JSO2 which had 7.2µg/ml of DNA. JSO2 showed better purity of the DNA ranging from 1.72 to 1.84 than JSO1 which had 1.69 to 2.15. JSO1 showed higher DNA growth than JSO2 but purity was seen higher in JSO2 than JSO1. Relationship between turbidity and DNA concentration was significant, but the level of significance was low suggesting DNA quantification may be useful to quantify the bacterial biomass.



Further details @ http://www.jocpr.com/

For more details to visit: http://www.jocpr.com/articles/quantification-of-bacterial-biomass-by-dna-analysis.pdf



Comments

Post a Comment

Popular posts from this blog

Quantitative analysis of civetone and normuscone in secretion from Viverricula indica and in aromatic remedies by gas chromatography-mass spectrometry

Image
ABSTRACT : The quantitation of civetone and normuscone in small Indian civet (Viverricula indica Desmarest) secretion cultivated in Thailand was analyzed by gas chromatography- mass spectrometry (GC-MS). Three main chemical constituents of small Indian civet secretion were civetone, dihydrocivetone and normuscone. The contents of chemical constituents were different between male and female secretion. Civetone (23.6 ± 1.5 µg/mg of secretion) was a major chemical constituent in the female secretion while normuscone (52.1 ± 5.9 µg/mg of secretion) was a major in the male secretion. Both civetone and normuscone were found in civet fur but not in the feces. Aromatic remedies which claimed to use the small Indian civet secretion as an ingredient showed both civetone and normuscone. Linearity range of civetone was 0-50 µg/ml with a correlation coefficient of 0.9717, and of normuscone was 0-80 µg/ml with a correlation coefficient of 0.9965. The average recoveries were 97.3-98.0% in sec...
Read more

Antimicrobial activity of aqueous, ethanolic, methanolic, cyclohexanic extracts and essential oil of Nigella sativa seeds

Image
  ABSTRACT : Most of the bacterial pathogens are resistant to existing synthetic antibacterial agents demanding an increasing effort to seek effective phytochemicals as antibacterial agents against such pathogens. Nigella sativa L. (black cumin) seeds play an important role in folk medicine and some of its major constituents are reported to be pharmacologically active. In this present work, Nigellasativa seed extracts were obtained using maceration extraction by various solvents (water, ethanol, methanol, and cyclohexan). The antibacterial and antifungal effects of different extracts and essential oil of seeds were investigated by the agar diffusion method against two gram positive (Bacillus subtilis CIP52.62 ,Staphylococcus aureus CIP4.83) and three gram negative strains of bacteria (Escherichia coli CIP53.126 ,Pseudomonas aeroginosa CIP82.118, Salmonella abony CIP80.39) and one strain of fungi (Candida albicans CIP48.72). All the extracts and essential oil showed varying d...
Read more

Analysis of total phenols,total tannins and screening of phytocomponents in Indigofera aspalathoides (Shivanar Vembu) Vahl EX DC

Image
ABSTRACT: The plant Indigofera aspalathoides is popularly known as Sivanar vembu in Tamil belongs to the family fabaceae. The stem is traditionally used for various skin disorders and cancer. In the traditional medicinal system, the leaves, flowers and tender shoot are said to be cooling and demulcent, they are used in the form of decoction for leprosy and cancer affections. Qualitative and quantitative Phytocomponents test were screened in Indigofera aspalathoides. The total content of some important phytochemical constituents like phenols, tannins of Indigofera aspalathoides by studied using spectrophotometric methods. The results showed that the plant is rich in phenols (47.38±1.532) compare than Tannins (34.59±1.788).  Further details Home page URL : http://www.jocpr.com/ For more details PDF Link :  http://www.jocpr.com/articles/analysis-of-total-phenolstotal-tannins-and-screening-of-phytocomponents-in-indigofera-aspalathoides-shivanar-vembu-vahl.pdf
Read more