UV-spectrophotometric method development and valida tion for estimation of Galantamine Hydrobromide in tablet do sage form

Abstract 

Two innovative, new, simple and low cost UV-spectrophotometric and first order derivative methods were developed and validated for estimationof Galantamine Hydrobromide in bulk drug and tablet dosage form. Galantamine Hydrobromide was estimated at 289 nm in Distilled Water. In first order derivative, it showed amplitude at 284.8 nm and λminima = 290.4 nm with 286.4 nm as zero crossing point (ZCP). In both the methods linearity was found to be in the range of 20-100 μg/ml; for UV spectrophotometric method (y = 0.007x - 0.002, r² = 0.999) and for first order derivative spectrophotometric method (Y=0.0012 x+0.00045; r2=0.999), respectively. These methods were analyzed and valiated for various validation parameters according to USP guidelines

Comments

Post a Comment

Popular posts from this blog

Validation of Spectrophotometric Methods for Quanti tative Determination of 7-ADCA in Pharmaceutical Formulations

Image
ABSTRACT Two simple, sensitive and accurate methods are described for the determination of 7-Amino deacetoxy cephalosporanic acid (7-ADCA) in bulk drug and in formulations. Method M 1 &M 2 are based on the oxidizing reaction between 7-ADCA and Ninhydrin-Ascorbic acid (NIN-AA) (M 1 ); β -Napthol-H 2 O 2 -Cupric nitrate-2, 4-DNP-Ammonium hydroxide ( β -NPT,H 2 O 2 -DNP)(M 2 ) solutions. The chromogen being extractable with chloroform could be measured quantitatively at 400 nm (M 1 ) and 420 nm (M 2 ).
Read more

Effect of conditions on the anodization of Zircaloy-4 in EDTA

ABSTRACT: Anodization of Zircaloy-4 in EDTA electrolyte has been studied under different anodizing conditions at room temperature. It has been observed that the change of current density and solvent has a marked influence on the kinetics of anodization. The kinetic parameters like rate of formation(dV/dt), ionic current efficiency(Ƞ) and differential field of formation(FD) were calculated. The surface natures of the anodized films were studied from Scanning Electron Micrographs(SEM) under different anodizing conditions. Further details to visit: http://www.jocpr.com/ For more details PDF Link:  http://www.jocpr.com/articles/effect-of-conditions-on-the-anodization-of-zircaloy4-in-edta.pdf
Read more

Method development and validation of RP-HPLC method for simultaneous determination of Lamivudine and Zidovudine

ABSTRACT: A rapid, sensitive and specific RP-HPLC [1-5] method involving UV detection was developed and validated for determination and quantification of Lamivudine and Zidovudine. Chromatography was carried out on a pre-packed AltimaC18 5µ (150*4.6mm) column using filtered and degassed mixture of Ammonium acetate buffer:Methanol (80:20) as mobile phase at a flow rate of 1.0ml/min and effluent was monitored at 270nm. The method was validated in terms of linearity, precision, accuracy, and specificity, limit of quantification and limit of detection. The assay was linear over the concentration range of Lamivudine and Zidovudine was 37.5mcg-112.5mcg/ml and 75mcg to 225mcg/ml respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 98.50%-99.9% and 98.30%- 100.10% within precision RSD of 0.71 and 0.82 for Lamivudine and Zidovudine respectively. The system suitability...
Read more