A process of method development: A chromatographic approach
Introduction
Prior to the initiation of method development, all the
known information about the analyte such as its structure, physical and
chemical properties, toxicity, purity, hygroscopicity, solubility, and stability
should be determined. These data may be available from preformulation reports,
early drug discovery sample screening reports, from the literature on similar
compounds, or from past experience with similar compounds. However, many times
this information is not available, so preventive measures must be taken in
order to ensure that the analyte does not degrade or change during the method
development scouting experiments. Implementation of a tray cooler, preparation
of fresh samples, storage of samples in a refrigerator, and protection of the
solids and sample solutions from light represent some common preventive
measures.
Comments
Post a Comment