Bioanalytical method for determination of Nevirapine in-vivo in resource constrained laboratories


Abstract 

Simple high-Performance Liquid Chromatographic method for determination of Nevirapine in human plasma is hereby reported. Carbamazepine was used as internal standard (IS). The internal standard and the drug were extracted into basified di-isopropyl ether, dried with a current of air using hair drier. The dried sample was reconstituted with 120 μ l of mobile phase followed by injection of 90 μ l of the reconstituted sample into the chromatograph. A reversed phase column C18 was used and the mobile phase consisted of 250ml acetonitrile and 800 ml phosphate Buffer (pH 7.5). Detection was achieved at 282nm and both NVP and IS were well separated from endogenous substances.

Comments

Post a Comment

Popular posts from this blog

Synthesis of Atenolol Impurities

Image
ABSTRACT: The aim of this research was to synthesis impurities of Atenolol viz. 2-(4-Hydroxy phenyl acetamide(Impurity A) , 2- [4-[[(2RS)oxiran-2-yl]methoxy]phenyl] acetamide (Impurity C) and 2-[4-[(2RS)-3-chloro-2-hydroxypropxy] phenyl]acetamide (Impurity D ) by very conventional method. Further details Home page URL:   http://www.jocpr.com/ For more details PDF Link :    http://www.jocpr.com/articles/synthesis-of-atenolol-impurities.pdf
Read more

Evaluation of acrylate-sunflower oil copolymer as viscosity index improvers for lube oils

Image
ABSTRACT: Copolymers of sunflower oil with methyl methacrylate and decyl acrylate were synthesized and characterized. Intrinsic viscosity and viscometric molecular weight were determined by using Huggins and Mark- Houwink equation respectively. Viscosity index (VI) of the additive doped base oils were evaluated and compared with that of the mineral base oils. VI values of the additive doped base oils depend on the nature of mineral base oils used and the type and concentration of VI improvers. For a comparison, respective homopolymers e.g. polymer of sunflower oil, poly(methyl methacrylate) and poly(decyl acrylate) were also prepared, characterized and evaluated in the similar fashion. For more details PDF Link:  ht tp://www.jocpr.com/articles/evaluation-of-acrylatesunflower-oil-copolymer-as-viscosity-index-improvers-for-lube-oils.pdf Further details:  h ttp://www.jocpr.com/
Read more

Hepatoprotective and antioxidant efficacy of Anethum graveolens linn in carbon tetrachloride induced hepatotoxicity in albino rats

Image
ABSTRACT: In the present study, the hepatoprotective and antioxidant efficacy of Anethum graveolens Linn was evaluated in CCl4 induced hepatotoxicity in albino rats. Hepatotoxicity was induced in albino rats by subcutaneous injection of carbon tetrachloride. Hepatoprotective efficiency of 100 mg/Kg wt of ethonalic extract of Anethum graveolens Linn was evaluated. Hepatotoxicity in experimental rats was evidenced by enhancement in the activity of SGOT, SGPT, MDA, & ALP and significant decrease in the activity of GSH, GPX, SOD & CAT. In plant extract treated animals, the toxicity effect of CCl4 was controlled significantly by restoration of the activity of above serum enzymes and antioxidant enzymes compared to the normal and standard drug silymarin treated groups. Therefore the results of present study support the hepatoprotective effect of Anethum graveolens Linn. For more details Home Page URL: http://www.jocpr.com/ Further detail PDF Link:  http://www.jocpr...
Read more