Bioanalytical method for determination of Nevirapine in-vivo in resource constrained laboratories


Abstract 

Simple high-Performance Liquid Chromatographic method for determination of Nevirapine in human plasma is hereby reported. Carbamazepine was used as internal standard (IS). The internal standard and the drug were extracted into basified di-isopropyl ether, dried with a current of air using hair drier. The dried sample was reconstituted with 120 μ l of mobile phase followed by injection of 90 μ l of the reconstitutedsample into the chromatograph. A reversed phase column C18 was used and the mobile phase consisted of 250ml acetonitrile and 800 ml phosphate Buffer (pH 7.5). Detection was achieved at 282nm and both NVP and IS were well separated from endogenous substances.

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