Comparison of Extracts of Cyphostemma Glaucophilla on Total Protein and Membrane Stabilisation

ABSTRACT
The chloroform, ethanol and water extracts of pulve rised dried leaves of Cyphostemma glaucophilla were used in this study. Three sets of inbred Wistar albino rats of either sex aged 7-9months and weighing 100-150g were fed on Top feed rat diet and allowed freeaccess to water before and during the experiment. For each extract, five groups A, B, C, D and E of five animals each were respectively served daily doses of saline [0.85% Nacl,5ml/kg],0.5,1.0,1.5 and 2.0mg/kg respectively extract for 14days using stomach tubes. Twenty four hours after the last administration, the animals were sacrificed by ether anaesthesia. Blood samples were drawn into heparinised tubes by cardiac puncture and centrifuged at 5000xg for 10minutes.The supernatant was used as plasma.

Comments

Post a Comment

Popular posts from this blog

Phytochemical investigation of the roots ofGrewia microcosLinn.

Image
ABSTRACT: The present study reports the phytochemical investigation of the roots of Grewia microcos linn. Seven Phytoconstituents have been reported namely 9,12- octadecadienoic acid; Ursolic acid; Stigmasterol; 6,4- dihydroxy-3-propen chalcone; Dioctyl phthalate; N-methyl-6-β-(1’,3’,5’-trienyl)-3-β-methoxyl-3-β-methyl piperidine; and Dibutyl phthalate have been reported for the first time from the roots of Grewia microcos. Further details Home page URL : http://www.jocpr.com/ For more details PDF Link : http://www.jocpr.com/articles/phytochemical-investigation-of-the-roots-of-grewia-microcos-linn.pdf
Read more

Synthesis of Atenolol Impurities

Image
ABSTRACT: The aim of this research was to synthesis impurities of Atenolol viz. 2-(4-Hydroxy phenyl acetamide(Impurity A) , 2- [4-[[(2RS)oxiran-2-yl]methoxy]phenyl] acetamide (Impurity C) and 2-[4-[(2RS)-3-chloro-2-hydroxypropxy] phenyl]acetamide (Impurity D ) by very conventional method. Further details Home page URL:   http://www.jocpr.com/ For more details PDF Link :    http://www.jocpr.com/articles/synthesis-of-atenolol-impurities.pdf
Read more

A Validated specific Stability-indicating RP-HPLC method for Aripiprazole and its related substances

Image
ABSTRACT : A validated specific stability indicating reversed-phase liquid chromatographic method was developed for the quantitative determination of Aripiprazole and its related substances in bulk samples, pharmaceutical dosage forms in presence of degradation products. Forced degradation studies were performed on bulk sample of Aripiprazole as per ICH prescribed stress conditions using acid, base, oxidative, thermal stress and photolytic degradation to show the stability indicating power of the method. Significant degradation was observed during basic stress and no degradation was observed in other stress conditions. The chromatographic method was optimized using the samples generated from forced degradation studies and the impurity spiked with Aripiprazole. Good resolution between the peaks corresponds to impuritiy-1, degradation products from the analyte were achieved on YMC PACK C18 column using the mobile phase consists a mixture of Sodium dihydrogen Orthophosphate dehydra...
Read more