Molecular and atomic spectrophotometry and high performance liquid chromatographic determination of metronidazole in dosage forms via complex formation with Au(III) and Hg(II) ions in Solutions


ABSTRACT:

Uv-visible spectrophotometry, flame atomic absorption spectrophotometry and HPLC techniques have been developed for the assay of metronidazole in bulk drug and in dosage forms via complex formation with Au(III) and Hg(II) ions in solutions after fixing maximum reaction conditions . In the first two techniques, chloroform was used as a solvent for the two complexes with extraction percentages (96.652,93.975%) respectively. Stability constants for the two extracted complexes were (1.697x104 , 5.118x105 )respectively. Beer's law was obeyed for (5-55), (2- 22µg/ml) for uv-visible and atomic spectrophotometric methods respectively. The HPLC determination for metronidazole and its complexes was performed simultaneously without extraction or separation step with peak absorption at λ(272nm), (280nm) for Au and Hg complexes respectively. A rectilinear relationship between two mean peaks area for metronidazole and its complexes with concentrations was observed in the range (10-110 µg/ml). The correlation coefficients, regression equations, detection limits and recoveries for the two complexes in the three methods were calculated. These methods have been successfully applied to assay of metronidazole in preparations.

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